F A Q


 


Frequently Asked Questions


 

Services/Analyses Related

 

 

How do I avail of ASL services?

What are the ASL services?

What analyses are available for soil samples?

What analyses are available for plant samples?

What analyses are available for water samples, extracts and solutions?

How much does an analysis cost?

I've already submitted the Experiment Information Sheet, what's next?

How will I know that my analysis is completed?

Where can I get the data from the analysis?

How long does it take to get results of analysis?

What are the terms and conditions for the requested services?
Does ASL accept samples from external customers?
Where can I find citation of ASL methods?
   

Sample Preparation

 

 

How much samples should be submitted for analysis?

Should samples be prepared before submitting it to ASL?

How should soil samples be prepared for analysis?

How should plant samples be prepared for analysis?

How should water, extracts and solution samples be prepared for analysis?

How should radiolabeled samples be prepared for counting?

 

 

Facilities Related

 

 

What are ASL's facilities?

How do I avail of ASL facilities?

Can I use the facilities at once after submitting the request?

Does ASL provide Personal Protective Equipment's (PPE) for the grinder operators?

 

 

Charge/Cost Related

 

 

How much does an analysis cost?

How do I get charged for the services that were availed?

Does ASL charge me for the use of the facilities?

 

 

 


 

Services/Analysis Related

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How do I avail of ASL services?

 

 

 

 

  1. Go to the ASL website, create an account with ASL.
  2. Click on Create New Project Folder. The system will create a new project ID number.  Fill-up required information on your new experiment information, fill up budget code field, etc..
  3. Click on New Request link and click on  Add Sample Analysis Request.
  4. Choose one type of sample for the analysis request, (e.g. plant), and provide other information needed. Click on Continue button.
  5. Select analysis(es) of request by ticking appropriate Analyses check button. Click on Continue button after the selection. The estimated cost and weight requirements buttons will appear for your reference. Click on Submit button to complete the online analysis request. 
  6. You will be notified by e-mail once your analysis request has been assigned with an ASL reference number.
  7. Using your ASL account, submit your sample information online. Sample ID Forms in MS Excel format are available for download here. Fill it up and save the accomplished .xls form in .csv file type (Comma Separated Value format) and upload using your ASL account.
  8. Print charge slip form when the command "Print charge slip form" appears.
  9. Submit physical samples with barcoded stickers to ASL together with your duly signed charge slip.
  10. ASL performs sample inspection and validation and schedule validated samples for analysis.
  11. You will be notified by email and/or by phone call once analysis report for your samples is ready for pick-up at ASL.

Note: If samples need drying or grinding, ASL has an available sample preparation facilities that can be used for a fee. The cost of use and schedule availability of the required sample preparation facilities can be requested by sending e-mail to  and attach relevant request forms that you may download here. Requests for facility and service are NOT available online.

 

     You may also refer to the flowchart below:

 

 

     

What are the available ASL services?

 

 

Analytical services

  1. Soil analysis
  2. Plant analysis
  3. Water, solution or soil extract analysis

Non-analytical services

  1. Liaison with the Philippine Nuclear Research Institute for radioisotope license
  2. Storage of radionuclides and equipment with sealed sources
  3. Personnel monitoring with respect to exposure to radioactivity
  4. Area monitoring with respect to radioactive contamination
  5. Radioactive waste management
  6. Decontamination (to remove contaminants)

 

 

What analyses are available for soil samples?

 

 

  1. pH
  2. Electrical Conductivity
  3. Organic Carbon
  4. Kjeldahl Nitrogen
  5. Exchangeable bases (Na,K,Ca,Mg,Al,H+)
  6. Cation Exchange Capacity
  7. Available nutrients (P, K, Zn, Cu, B, Cd)
  8. Active Fe and Mn
  9. Texture
  10. Total C & N, 13C and 15N

Understanding Soil Testing...

 

 

What analyses are available for plant samples?

 

 

  1. Kjeldahl N
  2. Total C & N, 13C and 15N
  3. ICP Macroelements (Na, K, Ca, Mg, P, S)
  4. ICP Microelements (Fe, Zn, Al, Cu, Mn, )
  5. AAS elements (Na, K, Ca, Mg, Fe, Zn, Cu, Mn)
  6. Boron by ICP-OES
  7. Crude SiO2
  8. Crude Ash
  9. Cd analysis by GF-AAS

 

 

What analyses are available for water samples, extracts and solutions?

 

 

  1. pH
  2. Electrical Conductivity
  3. NH4-N, NO3-N , Total N
  4. ICP elements (Na, K, Ca, Mg, P, S, Fe, Zn, Al, Cu, Mn, B, Si)

 

 

 

I've already submitted the Experiment Information Sheet, what's next?

 

 

  • You will be informed by e-mail on the status of your request.

 

 

How will I know that my analysis is completed?

 

 

  • ASL will notify by e-mail and by phone.

 

 

Where can I get the analysis report for my samples?

 

 
  • The duly signed hardcopy of the official Analysis Report and excess samples are picked up at ASL. You may also request an e-copy of your results by email to once the official Analysis Report and excess samples are picked up from ASL.

 

 
How long does it take to get results of analysis?  

 

  • This depends on ASL's current workload, type of analysis requested and the number of samples. Please contact    to  request for an estimate turnaround time of analysis or the date to expect results of analysis. If you need it faster, you may opt to request for RUSH samples with 30% cost surcharge.

 

 

What are the terms and conditions for the requested services?

 

 
  • All services are operated on a first come, first served basis. Every effort will be made to give adequate turnaround time of your samples.
  • RUSH samples are also accepted with 30% cost surcharge to cover up for the overtime work schedule for their analysis. A minimum number of 25 samples is required for RUSH analysis of samples to proceed.

 

ASL reserves the right to reject samples for analysis if there is evidence of:

  • sample deterioration or contamination or infestation,
  • incorrect sampling, or
  • improper drying or storage before sample submission

 

 
Does ASL accept samples from external customers?  

 

 

 

 

 


 

Sample Preparation

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How much samples should be submitted for analysis?

 

 

Minimum weight of samples to be submitted for Plant Analysis

 

Analysis

Weight of sample/analysis       

( g plant, 0.841 mm, No. 20 mesh)

Kjeldahl N

0.5

Wet ashing - ICP Macroelements

4.0

Wet ashing - ICP Microelements

4.0

Boron

1.0

Total C & N, 13C & 15N

2.0 (0.177mm, No. 80 mesh)

 

Minimum weight of samples to be submitted for Soil Analysis

 

Analysis

Weight of sample/analysis

( g soil, 2 mm ,# 10 mesh)

pH (by H2O, KCl, CaCl2)

40

EC

80

Olsen P

10

Bray P

20

CEC & Exchangeable Bases (Na,K,Ca,Mg)

20

Exchangeable Al & H+

40

Available K

20

PSA (Hydrometer)

240

PSA (Pipet)

50

Available Cu & Zn

40

Active Fe & Mn

2

Kjeldahl N

1

Organic C

5

Available B

40

 

 

Analysis

Weight of sample/analysis

( g soil, 0.177 mm, # 80 mesh)

Total C & N, 13C & 15N

1

 

Minimum weight of samples to be submitted for Water Analysis

 

  • Submit 50 ml of filtered *water, solution or extract samples in a glass or plastic container with a cap. Submit also at least 1 liter of matrix or extractant for calibrating standard solution preparation.

 

         For example, if you are submitting irrigation water as your sample, submit 1 liter of deionized or distilled water as your matrix. If your sample is a KCl extract, submit 1 liter of the same solution used in the extraction procedure.

 

  • Label samples with the following information:

 

 

Sample ID:  _________________

Sender: ____________________

Matrix or extractant used: ___________________

Who did the sampling/extraction:  _________________

When it was done: ________________

 

 

* filter water or solution sample with Whatman filter paper # 40

 

Should samples be prepared before submitting it to ASL?

 

 

  • Yes, read the FAQ for the specific sample.
  • The responsibility for correct sampling and sample preparation rests on the researcher. ASL reserves the right to reject samples for analysis if there is evidence of:
  1. sample deterioration or contamination or infestation,
  2. incorrect sampling, or
  3. improper drying or storage before submission
  • Samples from a foreign country should meet the importation requirements of the Plant Quarantine Service, Philippine Bureau of Plant Industry. Please refer to the Seed Health Unit Section for guidelines regarding import permits.

 

 

How should soil samples be prepared for analysis?

 

 

  • ASL Soil Processing Facilities are located at the Annex, ground floor level of the Kenzo Hemmi Bldg. All requests for the use of these facilities must be e-mailed to at least one week before the date of use.

 

1.       For pH, EC, exchangeable bases, CEC, particle size analysis, available P, K, available Zn & Cu, active Fe & Mn, organic C, Kjeldahl N, and B requiring coarse samples (2 mm ,# 10 mesh):

 

The soil required for normal analytical work is fine earth or soil that has been air-dried, crushed and passed through a 2-mm sieve to remove stones, twigs, leaves, stems and roots. Dried soil should be ground to 2 mm size using the Rukhuhia grinder (RG) located in Soil Grinding Room (Rm.148). (available at ASL).

 

Submit at least 600g of ground sample placed in an 8.5-size coin envelope (available at ASL), label envelope with the following information:

 

 

Sample ID: ______________

Sender: ____________________

Grinder used: ___________

Who did the grinding: _________________

When grinding was done: ________________

 

 

2.       For total C, C-13, total N and/or N-15 analysis requiring fine samples (0.177 mm, # 80 mesh) :

 

Using Pascall grinder, take an aliquot of the bulk sample for fine soil grinding. Once the soil has been ground to fine powder, it cannot be used for analyses listed in (1) above.

 

Submit at least 1g of fine sample placed in an 8.5-size coin envelope (available at ASL), label envelope following #1 procedure.

 

  • Once samples are ready for submission, customer may now avail of online Request for Analysis (RFA) using ASL website at http://asl.irri.org where forms for RFA, Sample IDs and Charge Slips are available. Fill out the required forms and submit to ASL using your ASL account. New customers should create an account with us first before availing of the online request system.

 

 

How should plant samples be prepared for analysis?

 

 

  • Plant Processing Facilities are located at the ground floor level of the Kenzo Hemmi Bldg. All requests for the use of these facilities must be e-mailed to at least one week before the date of use.

 

For Kjeldahl N analysis, macro- and micronutrient analysis by acid digestion and ICP-OES (sample size: 0.841 mm, No. 20 mesh)

 

Oven-dry samples at 80oC. Large dried samples are first ground using stainless steel beater cross grinder and subsampled by quartering to a manageable size. To obtain homogeneous powder, samples are finely ground using stainless steel cyclone Udy Mill or Wiley Mill and allowed to pass through a 20-mesh stainless steel sieve to avoid metallic contamination. After grinding, the whole sample must be mixed thoroughly.

 

In between samples, the mill is thoroughly cleansed with a stiff-bristled brush or compressed air. Additional step of sample pre-flushing is recommended before collecting the ground sample for analysis in order to avoid cross-contamination.

 

Submit at least 5g of ground sample placed in a coin envelop (available at ASL), label envelop with the following information:

 

 

Sample ID: ______________

Sender: ____________________

Grinder used: ___________

Who did the grinding: _________________

When grinding was done: ________________

 

 

For total C, C-13, total N and/or N-15 analysis by Dumas method ( sample size: 0.177mm, No. 80 mesh):

 

Grind oven-dried plant samples using stainless steel ball mill (SSBM) to talcum powder fineness. Ask ASL staff for assistance on how to use the SSBM. Submit at least 2 g of ground sample placed in a coin envelope (available at ASL), label coin envelope as in #1.

 

For Boron analysis by dry ashing and ICP-OES:

 

Samples must be oven-dried overnight at 60C to prevent B volatilization. If a sample is dried at 60C for B, it can be used for other determinations after drying at 80C. Follow the same grinding procedure as #2 and sample labeling as #1.

 

 

  • Once samples are ready for submission, customer may now avail of online Request for Analysis (RFA) using ASL website at http://asl.irri.org where forms for RFA, Sample IDs and Charge Slips are available. Fill out the required forms and submit to ASL using your ASL account. New customers should create an account with us first before availing of the online request system.

 

 

 

How should water, extracts and solution samples be prepared for analysis?

 

 

  • Submit 50 ml of filtered *water, solution or extract samples in a glass or plastic container with a cap. Submit also at least 1 liter of matrix or extractant for calibrating standard solution preparation.

 

         For example, if you are submitting irrigation water as your sample, submit 1 liter of deionized or distilled water as your matrix. If your sample is a KCl extract, submit 1 liter of the same solution used in the extraction procedure.

 

 

  • Label samples with the following information:

 

 

 

Sample ID: ______________

Sender: ____________________

Matrix or extractant used: ___________________

Who did the sampling/extraction: _________________

When it was done: ________________

 

 

 

  • Once samples are ready for submission, customer may now avail of online Request for Analysis (RFA) using ASL website at http://asl.irri.org where forms for RFA, Sample IDs and Charge Slips are available. Fill out the required forms and submit to ASL using your ASL account. New customers should create an account with us first before availing of the online request system.

 

 

How should radiolabeled samples be prepared for counting?

 

 

  • Liquid radiolabelled samples for counting should be treated with scintillant and submitted in liquid scintillation vials.

 

 


 

Facilities Related

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What are the ASL facilities?

 

 

  1. Plant washing room
  2. Plant drying room
  3. Plant grinding room
  4. Soil drying room
  5. Soil grinding room
  6. Radioisotope laboratory
  7. Autoradiography room
  8. Neutron and gamma probe storage room
  9. Radioactive waste storage facilities
  10. Wet laboratory for inorganic analysis

 

 

How do I avail of ASL facilities?

 

 

  • ASL's plant and soil sample preparation facilities are located at the ground floor level of the Kenzo Hemmi Bldg. All requests for the use of these facilities must be e-mailed to at least one week before the date of use. Appropriate request forms must be filled out and they could be downloaded here.

  • To avoid cross-contamination of plant and soil samples, separate sample preparation facilities are provided for each type of samples. Operators of the sample grinders should provide his/her dust mask and ear muffs for use during the grinding process. In addition, users are expected to exercise proper care of the facilities, observe good housekeeping, and leave the work areas and equipment clean when work is completed.

  • Radioisotope Laboratory (RL). The ASL provides facilities for handling and analysis of radionuclide materials as well as non-analytical services related to radiological safety.

 

 

Can I use the facilities at once after submitting the request?

 

 

  • You may use the facilities after receiving an approval schedule from ASL staff-in-charge.

 

 

Does ASL provide Personal Protective Equipment's (PPE) for the grinder operators?

 

 

  • No. PPE should be provided by the customer-in-charge for the operator.

 

  • PPE for grinder operators must include: dust mask, ear plugs, closed-toe shoes

 

 


 

Charge/Cost Related

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How do I get charged for the services that were availed?

 

 

  • When you submit samples, you are required to provide a budget code on the charge slip. ASL works with IRRI's accounting office for the processing of payments. The cost of ASL services is charged to the project specified by the researcher during the budget year in which the samples are submitted.

 

 

How much does an analysis cost?

 

 

Please click ASL cost calculator to view detailed pricing. For external customers, please request quotation from:

 

Does ASL charge me for the use of the facilities?

 

 

  • Effective January 01, 2013, ASL will charge customers for the use of ASL's sample preparation facilities. Please contact  for available facilities-cost per use details.

 

 




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